‘印度青’苹果合成与分解代谢通量研究及贮藏温度的优化

本研究以‘印度青’苹果为原料，研究在不同贮藏温度下合成与分解代谢网络通量，并在此基础上优化其贮藏温度。将‘印度青’苹果放置在7 个不同温度（0、2、5、10、15、20、40 ℃）条件下，测定‘印度青’苹果不同部位糖酵解途径、三羧酸循环、蔗糖合成途径、磷酸戊糖途径的代谢通量。结果显示：2 ℃时蔗糖有较高的合成通量，由外到内4 个部位分别为32、42、47和33，乳酸盐也在2 ℃时有较高的代谢通量，由外到内4 个部位分别为1.87、1.86、1.86和1.86，在此温度下有较低的分解代谢和较高的蔗糖合成代谢，因此，2 ℃是‘印度青’苹果较好的贮藏温度。通过不同温度下‘印度青’苹果的各个部位之间代谢通量上的变化，可以建立以乳酸盐与蔗糖通量为评价标准的定量化模型，这可为今后更好地研究延长水果的贮藏寿命提供新的研究方法和理论参考。

Anabolic and Catabolic Flux Analysis of ‘Indian Green’ Apples and Optimization of Storage Temperature

In this study, the anabolic and catabolic flux of ‘Indian Green’ apples were studied at different storage temperatures. Further, the storage temperature was optimized. The metabolic flux of the glycolytic pathway, trichloroacetic acid (TCA) cycle, sucrose synthesis pathway, and pentose phosphate pathway in different parts of the fruit were measured at storage temperatures of 0, 2, 5, 10, 15, 20, and 40 ℃. The results showed that sucrose had a higher anabolic flux at 2 ℃, with values of 32, 42, 47, and 33.00 for the four parts from the outside to the inside. Similarly, lactate also had a higher metabolic flux at 2 ℃, with values of 1.87, 1.86, 1.86, and 1.86 for the four parts from the outside to the inside. At this temperature, lower catabolism and higher sucrose anabolism were observed. Accordingly, 2 ℃ was the best storage temperature for ‘Indian Green’ apples. In this study, based on the changes in metabolic flux between various parts of ‘Indian Green’ apples at different storage temperatures, a quantitative model could be established to evaluate lactate and sucrose flux, which will provide a novel method and a theoretical reference for future studies to extend the storage life of fruits.