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基于高通量测序分析复配小曲白酒发酵过程中微生物群落结构及多样性
引用本文:韩国强,孙协平,吴鹏飞,陈今朝,陈春,王庆. 基于高通量测序分析复配小曲白酒发酵过程中微生物群落结构及多样性[J]. 食品科学, 2021, 42(18): 80-85. DOI: 10.7506/spkx1002-6630-20200513-152
作者姓名:韩国强  孙协平  吴鹏飞  陈今朝  陈春  王庆
作者单位:(长江师范学院现代农业与生物工程学院,重庆 408100)
基金项目:国家自然科学基金青年科学基金项目(31601460);重庆市技术创新与应用发展专项(cstc2019jscx-msxmX0351);重庆市教育委员会科学技术研究项目(KJQN201801429)
摘    要:利用高通量测序对复配小曲清香型白酒酿造过程中微生物多样性及变化规律进行比较分析.结果表明,样品中共检测出35个门、378个属的细菌和4个门、38个属的真菌.优势菌门为厚壁菌门(Firmicutes)、变形菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)、子囊菌门(Ascomycota)和毛霉...

关 键 词:复配小曲  高通量测序  细菌群落  真菌群落  微生物群落多样性

High Throughput Sequencing-based Analysis of Microbial Community Structure and Diversity during Baijiu Fermentation with Mixed-strain Xiaoqu
HAN Guoqiang,SUN Xieping,WU Pengfei,CHEN Jinzhao,CHEN Chun,WANG Qing. High Throughput Sequencing-based Analysis of Microbial Community Structure and Diversity during Baijiu Fermentation with Mixed-strain Xiaoqu[J]. Food Science, 2021, 42(18): 80-85. DOI: 10.7506/spkx1002-6630-20200513-152
Authors:HAN Guoqiang  SUN Xieping  WU Pengfei  CHEN Jinzhao  CHEN Chun  WANG Qing
Affiliation:(School of Advanced Agriculture and Bioengineering, Yangtze Normal University, Chongqing 408100, China)
Abstract:In this study, the diversity of microbial community during the fermentation of light-flavor Baijiu with mixed-strain Xiaoqu was analyzed by high-throughput sequencing. The results showed that a total of 35 bacterial phyla and 378 genera as well as four fungal phyla and 38 genera were detected. Firmicutes, Proteobacteria, Bacteroidetes, Ascomycota and Mucoromycota were the dominant phyla. The microbial community structure kept changing during the fermentation process. Unidentified Rickettsiales was the dominant genus over the first two days; Lactobacillus was the dominant genus from day 3 until the end of fermentation. The dominant fungal genera were Issatchenkia, Rhizopus, Saccharomyces and Aspergilus. The relative abundance of Rhizopus and Aspergilus decreased with the increase in fermentation time, while the relative abundance of Saccharomyces increased from day 2 onwards to become dominant. These results provide theoretical support for the brewing of Baijiu with mixed-strain Xiaoqu.
Keywords:mixed-strain Xiaoqu   high-throughput sequencing   bacterial community   fungal community   microbial community diversity,
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