果蔬中外泌体样纳米颗粒的分离、表征和应用研究进展

果蔬中的外泌体样纳米颗粒(exosome-like nanoparticles,ELNs)是由果蔬细胞释放的纳米级囊泡结构,与动物的外泌体具有类似的超微结构,但其脂质、蛋白质及核酸等化学成分具有显著差异,并具有独特的生物学功能.本文综述了2013年以来数十种果蔬中ELNs的相关研究进展,对其提取方法和表征手段进行了分析...

Isolation,Characterization and Application of Exosome-Like Nanoparticles from Fruits and Vegetables: A Review

The exosome-like nanoparticles (ELNs) in fruits and vegetables are nano-scale extracellular vesicles released by fruit and vegetable cells, with similar structures to the exosomes of mammal cells, and have significantly varying lipid, protein and nucleic acid profiles as well as unique biological functions. In this review, the progress that has been made in studies on the ELNs in dozens of fruits and vegetables since 2013 are summarized with a special focus on the methods used for ELNs extraction and characterization and on the differences in the chemical profiles including lipids, proteins, and nucleic acids of ELNs from different plant sources. Also, the potential health benefits and application as drug carriers of these ELNs are reviewed. This article is expected to provide enlightenments for further investigations on ELNs from fruits and vegetables.