| 极大螺旋藻藻蓝蛋白的提取与纯化 |
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| 引用本文： | 韦萍,李环,张成武.极大螺旋藻藻蓝蛋白的提取与纯化[J].陶瓷科学与艺术,1999(3). |
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| 作者姓名： | 韦萍 李环 张成武 |
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| 作者单位： | 南京化工大学制药与生命科学学院 |
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| 摘 要： | 将极大螺旋藻用高渗液低温浸泡、经（ＮＨ４）２ＳＯ４沉淀、透析后，经过改进的羟基磷灰石柱层析２次纯化得到试剂级的藻蓝蛋白。比较了干藻粉在不同高渗液的渗出液条件下藻蓝蛋白的得率和纯度。建立了低温浸泡时ＰＣ得率与纯度和时间的关系。提出了一种简易可行而高效的羟基磷灰尘石制法。
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| 关 键 词： | 极大螺旋藻 藻蓝蛋白 羟基磷灰石 |
EXTRACTION AND PURIFICATION OF PHYCOCYANINS FROM THE BLUEGREEN \%ALGAE SPIRULINA MAXIMA |
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| Wei Ping\ Li Huan\ Zhang Chengwu\= College of Life Science and Pharmacy,Nanjing University of Chemical Technology,\=Nanjing,China \=\=.EXTRACTION AND PURIFICATION OF PHYCOCYANINS FROM THE BLUEGREEN \%ALGAE SPIRULINA MAXIMA[J].Ceramics Science & Art,1999(3). |
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| Authors: | Wei Ping\ Li Huan\ Zhang Chengwu\= College of Life Science and Pharmacy Nanjing University of Chemical Technology \=Nanjing China \=\= |
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| Affiliation: | Wei Ping\ Li Huan\ Zhang Chengwu\= College of Life Science and Pharmacy,Nanjing University of Chemical Technology,\=Nanjing,210009,China \=\= |
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| Abstract: | After the algae being suspended in aqueous solutions of high inorganic salts at 4, ammonium sulphate precipitation, dialysis and modified hydrorylapatite chromatography were used effectively to achieve reagentgrade \%phycocyanin\%. The yield and purity of extracting \%phycocyanin\% from different aqueous solutions were compared, the relation of phycocyanin yield and purity and extraction time at low temperature was founded and a simple and effective preparation method of hydroxylapatite was introduced.\= |
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| Keywords: | spirulina maxima %phycocyanin\%\ hydroxylapatite |
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