| 重组大肠杆菌全细胞催化合成S-苷甲硫氨酸 |
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| 引用本文： | 牛卫宁,左晓佳,丁焰,尚晓娅,钦传光. 重组大肠杆菌全细胞催化合成S-苷甲硫氨酸[J]. 精细化工, 2009, 26(3) |
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| 作者姓名： | 牛卫宁 左晓佳 丁焰 尚晓娅 钦传光 |
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| 作者单位： | 西北工业大学,生命科学院,陕西,西安,710072 |
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| 基金项目： | 国家自然科学基金资助项目(20802057);;中国博士后科学基金项目(20070411144);;西北工业大学青年科技创新基金(W016212);;西北工业大学科技创新基金(W016143)~~ |
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| 摘 要： | 从大肠杆菌(E.ColiK12)基因组DNA中克隆出甲硫氨酸腺苷转移酶基因,构建了能高效表达甲硫氨酸腺苷转移酶的重组大肠杆菌E.ColiJM109(pBR322-MAT)。将重组大肠杆菌细胞用于催化合成S-腺苷甲硫氨酸(SAM)的研究中。实验发现,用φ(甲苯)=2%的水溶液对重组细胞进行通透化处理后,能大幅提高SAM的产率。探讨了底物浓度、温度、pH、反应时间以及菌体密度对反应转化率的影响。最佳反应条件为:底物浓度(ATP)30 mmol/L,反应温度35℃,pH=7.0,反应时间8 h,细胞密度80 g湿细胞/L反应液。在此条件下,底物ATP的转化率超过95%。
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| 关 键 词： | 全细胞催化 S-腺苷甲硫氨酸 大肠杆菌 重组细胞 医药与日化原料 |
Biosynthesis of S-adenosylmethionine by Whole Cell of Recombinant E.coli. |
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| NIU Wei-ning,ZUO Xiao-jia,DING Yan,SHANG Xiao-ya,QIN Chuan-guang. Biosynthesis of S-adenosylmethionine by Whole Cell of Recombinant E.coli.[J]. Fine Chemicals, 2009, 26(3) |
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| Authors: | NIU Wei-ning ZUO Xiao-jia DING Yan SHANG Xiao-ya QIN Chuan-guang |
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| Abstract: | The methionine adenosyltransferase gene was cloned from the genome of E.Coli K12,and the recombinant E.Coli JM109(pBR322-MAT) strain,which can highly express methionine adenosyltransferase was constructed.The optimal reaction conditions for the synthesis of S-adenosylmethionine(SAM) by the recombinant cells were investigated.The SAM yield can be greatly improved by using the recombinant cells treated with 2% toluene(volume fraction,water solvent) as the catalyst.The optimal conditions for the synthesis of S... |
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| Keywords: | whole cell catalysis S-adenosylmethionine Escherichia coli recombinant cell drug and cosmetic materials |
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