| 羊布鲁菌双抗体夹心ELISA检测方法的建立及验证 |
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| 引用本文： | 梅建军,王兴龙,李晓燕,刘锴,任林柱,贾剑锋,张琴. 羊布鲁菌双抗体夹心ELISA检测方法的建立及验证[J]. 中国生物制品学杂志, 2009, 22(8) |
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| 作者姓名： | 梅建军 王兴龙 李晓燕 刘锴 任林柱 贾剑锋 张琴 |
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| 作者单位： | 梅建军(内蒙古包头医学院基础医学部,内蒙古包头,014010);王兴龙,李晓燕,刘锴,任林柱(军事医学科学院军事兽医研究所,长春,130062);贾剑锋,张琴(内蒙古乌拉特中旗人民医院,内蒙古乌拉特中旗,015300) |
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| 摘 要： | 目的建立羊布鲁菌双抗体夹心ELISA检测方法,并进行验证。方法应用针对羊布鲁菌O链M抗原的种特异性单克隆抗体,建立检测羊布鲁菌抗原的双抗体夹心ELISA方法,并进行重复性、特异性、敏感性验证及初步应用。结果经验证,该方法重复性好,特异性强,最低检出限为3.0×103CFU/ml,与平板凝集试验的符合率为100%。结论已建立了特异、敏感的检测羊布鲁菌的双抗体夹心ELISA方法。
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| 关 键 词： | 羊布鲁菌 O链 M抗原 双抗体夹心ELISA |
Development and Verification of Double Antibody Sandwich ELISA for B. melitensis |
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| Abstract: | Objective To develop an ELISA method for detection of B.melitensis.Methods A double antibody sandwich ELISA method was developed with specific antibody against M antigen of B.melitensis O chain,then verified for reproducibility,specificity and sensitivity,and applied primarily.Results The developed ELISA method showed good reproducibility and high specificity,and its minimum detection limit was 3.0×103 CFU/ml.The coincidence rate of detection results by the developed method and that by plate agglutination test was 100%.Conclusion A specific and sensitive double antibody sandwich ELISA method for B.melitensis was developed. |
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| Keywords: | B.melitensis O chain M antigen Double antibody sandwich ELISA |
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