| 考马斯亮蓝法测定乳与乳制品中蛋白质含量 |
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| 引用本文： | 冯昕,王吉中,尧俊英,呼玉侠. 考马斯亮蓝法测定乳与乳制品中蛋白质含量[J]. 粮食与食品工业, 2010, 17(3): 57-59 |
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| 作者姓名： | 冯昕 王吉中 尧俊英 呼玉侠 |
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| 作者单位： | 1. 郑州轻工业学院食品与生物工程学院,郑州,450002
2. 上海奇泓生物科技有限公司郑州分公司,郑州,450013 |
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| 摘 要： | 建立了一种测定乳与乳制品中蛋白质含量的方法。利用6份乳与乳制品作为试验材料,通过考马斯亮蓝比色法测定其蛋白质含量。结果表明,工作曲线在0~0.18 g/100 mL范围内线性关系良好,线性相关系数为0.999 3,平均加标回收率为98.13%,相对标准偏差小于5%,方法的检出限为0.02 g/100 mL,该方法具有操作简便、重现性好、准确可靠等特点。
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| 关 键 词： | 考马斯亮蓝法 乳与乳制品 蛋白质含量 |
Determination of protein content in milk and dairy products by Coomassie Brilliant Blue method |
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| Feng Xin,Wang Jizhong,Yao Junying,Hu Yuxia. Determination of protein content in milk and dairy products by Coomassie Brilliant Blue method[J]. Cereal and Food Industry, 2010, 17(3): 57-59 |
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| Authors: | Feng Xin Wang Jizhong Yao Junying Hu Yuxia |
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| Affiliation: | 1. College of Food and Biology Engineering, Zhengzhou University of Light Industry (Zhengzhou 450002) ; 2. Zhengzhou Filiale of Shanghai Chihong Biotechnological Company (Zhengzhou 450013) ) |
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| Abstract: | A determination method of protein content of the milk and dairy products is established. Six milk and dairy products were taken as test material by Coomassie Brilliant Blue colorimetric to determinate protein content. The results show that the working curve in a range of 0- 0.18 g/100 mL leads a good linear relationship, the linear correlation coefficient is 0. 999 3, the average recovery is 98.13%, the relative standard deviation is less than 5 %, the detection limit is 0.02 g/100 mL and the method is simple, reproducible, accurate and reliable. |
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| Keywords: | Coomassie Brilliant Blue method milk and dairy products protein content |
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