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Chemical,antioxidant and antibacterial study of Brazilian fruits
Authors:Charles Windson Isidoro Haminiuk  Manuel Salvador Vicente Plata‐Oviedo  Amanda Roman Guedes  Ana Paula Stafussa  Evandro Bona  Solange Teresinha Carpes
Affiliation:1. Universidade Tecnológica Federal do Paraná, Programa de Pós‐Gradua??o em Tecnologia de Alimentos (PPGTA), Campus Campo Mour?o, PR, Brazil;2. Universidade Tecnológica Federal do Paraná, Campus Pato Branco, PR, Brazil
Abstract:In this study, the antioxidant capability, total phenolic content and antimicrobial activity of ethanolic extracts of seven fruits from the Brazilian Atlantic Forest were evaluated. The conditions for the extraction of crude phenolics from the fruits were determined using an experimental factorial design. Total phenolic content, 1,1‐diphenyl‐2‐picrylhydrazyl radical (DPPH?) scavenging activity and β‐carotene‐linoleic acid couple oxidation assays were used to evaluate the antioxidant properties of the extracts. In addition, antimicrobial activity was screened using two Gram‐negative bacteria (Escherichia coli and Klebsiella pneumoniae) and one Gram‐positive bacterium (Staphylococcus aureus). All native fruits assayed in this study have high potential as natural antioxidant sources. Among the seven fruits evaluated, Jabuticaba and Uvaia had the highest antioxidant activity in the DPPH? and of β‐carotene‐linoleic acid coupled oxidation assays. In the biological assay, K. pneumoniae was the most sensitive microorganism to the fruit extracts, and the Jabuticaba extract had a slight inhibitory effect against this Gram‐positive bacterium.
Keywords:Antimicrobial activity  1  1‐diphenyl‐2‐picrylhydrazyl radical  extraction  fruit extracts  phenolic compounds
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