Multiplex PCR for the simultaneous detection of Salmonella spp. and Salmonella Enteritidis in food |
| |
| Authors: | Daniel Santos Pinto Silva Thayse Canato Marciane Magnani Juliane Alves Elisa Yoko Hirooka Tereza Cristina Rocha Moreira de Oliveira |
| |
| Affiliation: | Universidade Estadual de Londrina, Centro de Ciências Agrárias, Departamento de Ciência e Tecnologia de Alimentos, Campus Universitário, C.P. 6001, CEP 87051‐970 Londrina, PR, Brasil |
| |
| Abstract: | Multiplex PCR assay (mPCR) for the detection of Salmonella spp. and S. Enteritidis was developed in this study using artificially contaminated chicken carcasses. The assay showed 100% specificity to detect approximately 1 CFU of Salmonella in 10 g of chicken skin after non‐selective enrichment. The mPCR was evaluated in Minas cheese, fresh pork sausage and chicken carcasses commercially available. Salmonella spp. was detected in nine of sixty‐six chicken carcasses, five of fifty‐two cheese samples, and five of fifty‐two sausage samples. The serovar Enteritidis was detected in two samples of contaminated sausage. The mPCR results were confirmed by conventional culture and biochemical identification of the isolates. Serotyping confirmed the presence of S. Enteritidis in sausage samples and showed contamination by serovars Schwarzengrund and Montevideo in chicken carcasses. |
| |
| Keywords: | Chicken carcasses multiplex PCR pork sausages Salmonella Enteritidis Salmonella spp. |
|
|
