Lysyl oxidase from jumbo squid (Dosidicus gigas) muscle: detection and partial purification |
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| Authors: | Wilfrido Torres‐Arreola Ciria G Figueroa‐Soto Elisa M Valenzuela‐Soto Guillermina Garcia‐Sanchez Enrique Marquez‐Rios Ramón Pacheco‐Aguilar |
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| Affiliation: | 1. Centro de Investigación en Alimentación y Desarrollo, A.C. Carretera a la Victoria Km 0.6. Apdo. Postal 1735. C.P. 83000, Hermosillo, Sonora, Mexico;2. Departamento de Investigación y Posgrado en Alimentos, Universidad de Sonora, Blvd. Luis Encinas y Rosales s/n. Apdo. Postal 1658, C.P. 83000 Col. Centro, Hermosillo, Sonora, Mexico |
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| Abstract: | Lysyl oxidase (LOX) was detected and partially purified from jumbo squid (Dosidicus gigas) muscle, for the first time. A procedure for the purification of LOX from jumbo squid muscle which consisted of urea extraction, Sephadex G‐75 and anion exchange chromatography was developed. Activity of partially purified LOX was 390‐fold higher than the original extract. Two protein fractions with 32 and 24 kDa were detected by SDS‐PAGE. The enzyme was strongly inhibited by β‐aminopropionitrile fumarate, a specific LOX inhibitor. LOX was purified with 3.8% yield, showing a specific activity of 0.078 IU mg?1 protein. This knowledge will help understand the behaviour of jumbo squid protein during cool storage or manufacture. |
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| Keywords: | Jumbo squid lysyl oxidase purification tentacles |
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