| 紫甘薯多酚氧化酶最适底物研究 |
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| 引用本文： | 高路.紫甘薯多酚氧化酶最适底物研究[J].粮食与油脂,2010(10). |
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| 作者姓名： | 高路 |
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| 作者单位： | 沈阳师范大学职业技术学院,辽宁沈阳,110036 |
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| 摘 要： | 紫薯多酚氧化酶分别以苯酚、愈创木酚、儿茶酚、L–酪氨酸、绿原酸、焦性没食子酸和间苯三酚这7种酚类物质为底物时,米氏常数Km分别为15.68、15.01、12.06、17.12、10.23、13.22、13.46 mmol/L;紫薯PPO最适作用底物为绿原酸,次适作用底物为儿茶酚。
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| 关 键 词： | 紫薯 多酚氧化酶 米氏常数 |
Study on optimum substrate of polyphenol oxidase from purple sweet potato |
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| GAO Lu.Study on optimum substrate of polyphenol oxidase from purple sweet potato[J].Cereals & Oils,2010(10). |
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| Authors: | GAO Lu |
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| Abstract: | The Michaelis constant(sKm)for 7 kinds of phenolic substrates,including phenol,guaiacol,catechol,L–tyrosine,chlorogenic acid,pyrogallic acid and 1,3,5–hydroxybenzene,are determined by Hanes–Woolf diagram.They are 15.68,15.01,12.06,17.12,10.23,13.22 and 13.46 mmol/L.The optimum substrate identified for PPO from purple sweet potato is chlorgenic acid,then catechol. |
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| Keywords: | purple sweet potato polyphenol oxidase michaelis constant |
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