| 一种胞外单宁酶的活力检测方法 |
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| 引用本文： | 保玉心,邱树毅,李秧针,黄永光.一种胞外单宁酶的活力检测方法[J].精细化工,2008,25(6):621-624. |
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| 作者姓名： | 保玉心 邱树毅 李秧针 黄永光 |
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| 作者单位： | 1. 贵州省发酵工程与生物制药重点实验室,贵州,贵阳,550003;贵州大学,化工学院,贵州,贵阳,550003;贵州大学,生命科学学院,贵州,贵阳,550025
2. 贵州省发酵工程与生物制药重点实验室,贵州,贵阳,550003;贵州大学,化工学院,贵州,贵阳,550003 |
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| 摘 要： | 介绍了一种胞外单宁酶的活力检测方法。根据绕丹宁与没食子酸在碱性条件下显色的原理,在520 nm波长下测定吸光值,避开了胞外单宁酶粗酶液中干扰物质的吸收峰波长,建立了一种适合固体发酵所产胞外单宁酶的酶活检测方法。
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| 关 键 词： | 单宁酶 胞外酶 固体发酵 |
| 文章编号： | 1003-5214(2008)06-0621-04 |
| 修稿时间： | 2007年11月30 |
A Method for Detecting Activity of Extracellular Tannase |
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| BAO Yu-xin,QIU Shu-yi,LI Yang-zhen,HUANG Yong-guang.A Method for Detecting Activity of Extracellular Tannase[J].Fine Chemicals,2008,25(6):621-624. |
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| Authors: | BAO Yu-xin QIU Shu-yi LI Yang-zhen HUANG Yong-guang |
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| Abstract: | A method for detecting the activity of extracellular tannase was introduced.According to the coloration reaction between tannase and gallic acid under alkaline condition,the detective wavelengths was set at 520 nm to avoid the absorption peak of interfering substances in crude extracellular tannase solution successfully.A proper method for detecting enzyme activity of extracellular tannase in solid fermentation was founded. |
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| Keywords: | tannase extracellular enzyme solid fermentation |
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