微波辅助双水相提取盾叶薯蓣中的皂苷成分

采用微波辅助乙醇/硫酸铵双水相体系，从盾叶薯蓣中提取皂苷成分. 实验结果表明，各种皂苷成分可有效地富集于醇相，盾叶新苷、三角叶皂苷、薯蓣皂苷元-葡萄糖三糖苷、薯蓣皂苷元-葡萄糖二糖苷、延龄草次苷的最大分配系数分别达89, 91, 93, 117和153. 微波辅助双水相提取皂苷成分的最佳药材/乙醇质量比为1:21，最佳相组成为乙醇25%(w)，硫酸铵19%(w)，该条件下的提取率为，总皂苷95.1%，盾叶新苷99.8%，三角叶皂苷97.4%，薯蓣皂苷元-葡萄糖三糖苷97.3%，薯蓣皂苷元-葡萄糖二糖苷86.6%，延龄草次苷63.2%.

Microwave-assisted Aqueous Two-phase Extraction of Steroidal Saponins in Dioscorea zingiberensis

Microwave-assisted aqueous two-phase extraction of steroidal saponins in Dioscorea zingiberensis was studied by ethanol/ammonium sulphate system. The results showed that almost all the five main saponins were enriched in the top phase, and the maximum partition coefficients of Zingibernsis newsaponin, deltonin, Diosgenin-triglucoside, Diosgenin-diglucoside and trillin were 89, 91, 93, 117 and 153, respectively. The optimum mass ratio of material to ethanol was 1:21. The optimum system was composed of 25%(ω) ethanol and 19%(ω) (NH_4)_2SO_4. Under the optimum condition, the yield of total saponins was 95.1%, and the yields of Zingibernsis newsaponin, deltonin, Diosgenin-triglucoside, Diosgenin-diglucoside and trillin were 99.8%, 97.4%, 97.3%, 86.6% and 63.2%, respectively.