Monitoring nisin desorption from a multi-layer polyethylene-based film coated with nisin loaded HPMC film and diffusion in agarose gel by an immunoassay (ELISA) method and a numerical modeling |
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| Authors: | E Chollet Y Swesi P Degraeve I Sebti |
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| Affiliation: | 1. Dept. of Seafood Processing, Faculty of Marine Sciences, Tarbiat Modares University, Noor 46414, Iran;2. Dept. of Food Science and Technology, National Nutrition and Food Technology Research Institute, Faculty of Nutrition and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran;1. Department of Polymer Science and Engineering, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, PR China;2. State Key Laboratory of Power Transmission Equipment and System Security and New Technology, Chongqing University, Chongqing, 400030, PR China;3. State Key Laboratory of Advanced Transmission Technology, Global Energy Interconnection Research Insititute, Beijing 102211, PR China;4. Department of Electronics and Computer Science, University of Southampton, Southampton SO17 1BJ, United Kingdom;5. State Key Laboratory of Power System, Department of Electrical Engineering, Tsinghua University, Beijing 100084, PR China;1. National Council of Scientific and Technical Research-CONICET, Av. Rivadavia 1917 (C1033AAJ), Buenos Aires, Argentina;2. National University of San Martín – UNSAM 3iA. Campus Miguelete, Av. 25 de Mayo y Francia (CP 1650), San Martín, Buenos Aires, Argentina;3. National Institute of Industrial Technology-INTI, Av. Gral. Paz 5445, San Martín (B1650WAB), Buenos Aires, Argentina;1. Novel Materials and Nanotechnology Group, IATA, CSIC, Paterna 46980, Spain;2. Dept. Prev Med, Faculty of Pharmacy, Univ. of Valencia, Burjassot 46100, Spain;1. Key Laboratory of Bio-based Material Science and Technology (Ministry of Education), College of Material Science and Engineering, Northeast Forestry University, 26 Hexing Road, Harbin 150040, PR China;2. College of Materials and Energy, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, PR China |
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| Abstract: | Polyethylene-based films coated with nisin loaded HPMC films were put in contact with food simulants, i.e. agarose gels with 5 or 30% (w/w) fat. Nisin desorption from the multi-layer films and diffusion in agarose gels were monitored by ELISA (Enzyme Linked ImmunoSorbent Assay). The data obtained after 2 or 6 days of contact between antimicrobial films and agarose gels were employed to determine nisin mass transfer by numerical modeling following Fick's second law. The values were in the range from 0.87 × 10? 3 m s? 1 to 4.30 × 10? 3 m s? 1 and 6.5 × 10? 11 m2 s? 1 to 3.3 × 10? 10 m2 s? 1, for nisin apparent desorption and diffusion coefficients, respectively. The diffusion process was governed by interactions between food matrix simulant and nisin. Moreover, it was observed that the polymer in the coating did not modify plastic film initial mechanical resistance and water vapor permeability.Industrial relevanceThis paper concerns active packaging, considered as a new approach to preserve food shelf life. Active packaging is a real gain for plastic and Food industrials. The paper deals with coating as a manner to activate packaging. The impact of coating on film properties is investigated.Also, predictive models are proposed to determine antimicrobial agent desorption and diffusion during some storage conditions. |
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