| 酿酒酵母胞内代谢关键酶对乙醇的耐受性 |
| |
| 引用本文： | 聂妤,董亮,侯德文,李明达,王晓丹,赵长新. 酿酒酵母胞内代谢关键酶对乙醇的耐受性[J]. 大连轻工业学院学报, 2011, 0(5) |
| |
| 作者姓名： | 聂妤 董亮 侯德文 李明达 王晓丹 赵长新 |
| |
| 作者单位： | 大连工业大学生物工程学院;华润雪花啤酒(盘锦)有限公司; |
| |
| 基金项目： | “十一五”国家科技支撑计划项目(2007BAK36B01) |
| |
| 摘 要： | 分别添加2%、4%、6%、8%乙醇的酵母细胞糖酵解途径和三羧酸循环中葡萄糖-6-磷酸脱氢酶、丙酮酸激酶、异柠檬酸脱氢酶、苹果酸脱氢酶、乙醇脱氢酶、异柠檬酸裂解酶活力等关键代谢节点处的酶活力,以期考查关键酶对酒精的耐受性。实验结果表明,葡萄糖-6-磷酸脱氢酶对酒精的耐受能力较强,添加8%乙醇时的酶活力较无乙醇时提高23.65%;丙酮酸激酶、异柠檬酸脱氢酶和乙醇脱氢酶表现出对酒精的耐受力较差,丙酮酸激酶和乙醇脱氢酶在添加2%乙醇时的酶活力较无乙醇时分别降低77.7%和62.72%,异柠檬酸脱氢酶在添加8%乙醇时的酶活力仅为0.003U;苹果酸脱氢酶和异柠檬酸裂解酶表现出对酒精良好的耐受性,在添加8%乙醇时的酶活力较无乙醇时分别提高256.25%和276.16%。
|
| 关 键 词： | 酿酒酵母 酶活力 乙醇 耐受性 |
Tolerance of key metabolic enzymes on ethanol in the vivo cell of Saccharomyces cerevisiae |
| |
| NIE Yu,DONG Liang,HOU De-wen,LI Ming-da,WANG Xiao-dan,ZHAO Chang-xin .School of Bioengineering,Dalian Polytechnic University,Dalian ,China,.China Resources Snow Breweries Company Limited,Panjin. Tolerance of key metabolic enzymes on ethanol in the vivo cell of Saccharomyces cerevisiae[J]. Journal of Dalian Institute of Light Industry, 2011, 0(5) |
| |
| Authors: | NIE Yu DONG Liang HOU De-wen LI Ming-da WANG Xiao-dan ZHAO Chang-xin .School of Bioengineering Dalian Polytechnic University Dalian China .China Resources Snow Breweries Company Limited Panjin |
| |
| Affiliation: | NIE Yu1,DONG Liang1,HOU De-wen2,LI Ming-da1,WANG Xiao-dan1,ZHAO Chang-xin1 1.School of Bioengineering,Dalian Polytechnic University,Dalian 116034,China,2.China Resources Snow Breweries(Panjin) Company Limited,Panjin 124010 |
| |
| Abstract: | The activity of Glucose-6-phosphate dehydrogenase,pyruvate kinase,isocitrate dehydrogenase,malate dehydrogenase,ethanol dehydrogenase and isocitrate lyase in the EMP pathway and TCA cycle were detected in the medium with 2%,4%,6%,8% ethanol to analyze the tolerance of key metabolic enzymes on ethanol in the vivo cell of Saccharomyces cerevisiae.The results show that glucose-6-phosphate dehydrogenase activity was increased by 23.65% when adding 8% ethanol;the activity of pyruvate kinase and alcohol dehydroge... |
| |
| Keywords: | Saccharomyces cerevisiae enzyme activity alcohol tolerance |
| 本文献已被 CNKI 等数据库收录！ |
|
