| 牡蛎糖原的提取、纯化及补体活性研究 |
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| 引用本文： | 宋荪阳,朱蓓薇,杨静峰,孙黎明.牡蛎糖原的提取、纯化及补体活性研究[J].大连轻工业学院学报,2011(5). |
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| 作者姓名： | 宋荪阳 朱蓓薇 杨静峰 孙黎明 |
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| 作者单位： | 大连工业大学海洋食品教育部工程研究中心;大连工业大学农业部农产品加工技术研发贝类专业分中心; |
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| 基金项目： | 国家高技术研究发展计划(863计划)项目(2011AA100803); 国家农转资金项目(2009GB2B000064); 辽宁省重点实验室项目(2008403003); 大连市科技攻关技术项目(2009B12NC014) |
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| 摘 要： | 采用酶解结合醇沉法从新鲜牡蛎中提取制备牡蛎糖原(oyster glycogen,OG),经阴离子交换柱DEAE-52、葡聚糖凝胶柱Sephadex G-100分离纯化,得到纯度较高的牡蛎糖原组分OG11。其蛋白质量分数为0.33%,糖质量分数为97.77%。用气相色谱法对OG11进行单糖组成鉴定,结果表明其中只含有葡萄糖。补体实验表明,OG11对补体具有较强的抑制活性,在5.0mg/mL时补体抑制率可达到77.38%。
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| 关 键 词： | 牡蛎糖原 提取 纯化 补体活性 |
Isolation and complement activity of glycogen from oyster glycogen |
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| SONG Sun-yang,ZHU Bei-wei,YANG Jing-feng,SUN Li-ming, .Engineering Research Center of Seafood of Ministry of Education,Dalian Polytechnic University,Dalian ,China,.Special Sub-center of Shellfish of National Research , Development Center of Agriculture Product Processing Technology of Ministry of Agriculture.Isolation and complement activity of glycogen from oyster glycogen[J].Journal of Dalian Institute of Light Industry,2011(5). |
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| Authors: | SONG Sun-yang ZHU Bei-wei YANG Jing-feng SUN Li-ming Engineering Research Center of Seafood of Ministry of Education Dalian Polytechnic University Dalian China Special Sub-center of Shellfish of National Research Development Center of Agriculture Product Processing Technology of Ministry of Agriculture |
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| Affiliation: | SONG Sun-yang1,2,ZHU Bei-wei1,YANG Jing-feng1,SUN Li-ming1,2 1.Engineering Research Center of Seafood of Ministry of Education,Dalian Polytechnic University,Dalian 116034,China,2.Special Sub-center of Shellfish of National Research and Development Center of Agriculture Product Processing Technology of Ministry of Agriculture |
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| Abstract: | Oyster glycogen(OG) was extracted by enzymatic hydrolysis and precipitation with alcohol.The extracted OG was purified to homogeneity by a combination of anion exchange chromatography(DEAE-52) and gel filtration chromatography(Sephadex G-100) to obtain high purity OG11,containing 97.77% polysaccharide and 0.33% protein.The gas chromatographic results indicated that oyster glycogen OG11 was a hompolysaccharide containing only glucose.OG11 showed a dose-dependent anti-complement activity in vitro,the maximal ... |
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| Keywords: | oyster glycogen isolation purification complement activity |
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