Absorption and biliary secretion of intraperitoneally injected 3β-methoxycholest-5-ene-4-14C in the rat

Following ip injection of 1 mg amounts of methoxycholestene-4-¹⁴C into normal intact rats, the amount of label in the whole liver was measured at different time intervals in different animals. These studies indicated that most of the injected compound was absorbed by or before 12 hr. In bile duct-cannulated rats the secretion of label from ip injected methoxycholestene-4-¹⁴C or cholesterol-4-¹⁴C into bile was usually less during the first 24 hr than during the second 24 hr after injection. Studies on the nature of the labeled compounds in the bile following ip injection of methoxycholestene-4-¹⁴C indicate that most of the label is present in metabolites, 47% of the label in cholic acid and 31% in chenodeoxycholic acid.