Immobilization of β‐Glucosidase BglC on Decanedioic Acid‐Modified Magnetic Nanoparticles |
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| Authors: | Qiang Fu Hongling Wang Zheng Zhang Ze Haji Xiutong Li Xinglong Lian Yingfeng An |
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| Affiliation: | 1. Forestry Department of Liaoning Province, Qingshan Protection Bureau, Shenyang, China;2. Shenyang Agricultural University, College of Biosciences and Biotechnology, Shenyang, China;3. Shenyang Agricultural University, College of Food Science, Shenyang, China |
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| Abstract: | β‐Glucosidase BglC from Thermobifida fusca was efficiently immobilized on decanedioic acid‐modified magnetic nanoparticles (DMNP). Interestingly, the immobilized BglC showed much higher organic solvent stability than the free enzyme, indicating that this enzyme immobilized on DMNP should be especially suitable for catalyzing reactions in organic solvents. Additionally, the immobilized BglC also showed better thermal stability, storage stability, and mechanical stability than the free counterpart. Moreover, the reuse times were satisfactory and even crude plant materials (e.g., rice husk and corn cob) were used as substrates, which should be valuable for the decomposition of natural organic materials and the full utilization of bioresources. |
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| Keywords: | Biodegradation β ‐Glucosidase Immobilized enzymes Magnetic nanoparticles |
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