| 霍乱弧菌噬菌体在消除海产品中相关弧菌的潜在应用研究 |
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| 引用本文： | 蔡俊鹏,王沛贤.霍乱弧菌噬菌体在消除海产品中相关弧菌的潜在应用研究[J].现代食品科技,2009,25(12):1375-1379. |
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| 作者姓名： | 蔡俊鹏 王沛贤 |
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| 作者单位： | 华南理工大学轻工与食品学院,广东广州,510640 |
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| 基金项目： | 国家自然科学基金40776091;广东省海洋渔业科技推广专项项目A200699H02 |
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| 摘 要： | 噬菌体具有裂解病菌的作用,可用作生物净化因子.本实验以霍乱弧菌为宿主菌,采用双层平板法从鲍鱼养殖环境中分离到5株霍乱弧茵噬菌体.以23株弧菌为宿主菌,本文研究了霍乱弧菌噬菌体对海产品中常见弧茵的裂解消除能力,同时针对宽裂解谱噬菌体进行包括热失活、pH稳定性、紫外照射、不同镁离子浓度、不同柠檬酸钠浓度等理化因素对其裂解能力的影响,以探索其裂解消除弧茵的最优条件.实验结果表明噬菌体在消除霍乱弧菌方面有潜在的应用价值,紫外照射、柠檬酸钠对噬菌体裂解有不同程度的抑制作用,温度和pH值对噬茵体裂解也有不同程度的影响,而适度的镁离子则有促进作用.
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| 关 键 词： | 噬茵体 霍乱弧菌 弧菌 裂解能力 理化因素 |
Lysis Study of Bacteriophages of Vibrio cholera on Seafood-Borne Potentially Pathogenic Vibrios |
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| CAI Jun-peng,WANG Pei-xian.Lysis Study of Bacteriophages of Vibrio cholera on Seafood-Borne Potentially Pathogenic Vibrios[J].Modern Food Science & Technology,2009,25(12):1375-1379. |
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| Authors: | CAI Jun-peng WANG Pei-xian |
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| Affiliation: | CAI Jun-peng,WANG Pei-xian(College of Light Industry and Food Sciences,South China University of Technology,Guangzhou 510640,China) |
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| Abstract: | It is a fact that bacteriophage can lyse Vibrio cholerae.In this study,5 strains of bacteriophage of Vibro cholerae using Sh10(Vibro cholerae) as a host bacterium,were respectively isolated from abalone farm in Shanwei,Guangdong,in 2008.Using 23 strains of vibrio as host,bacteriophages of Vibrio cholera lysis on seafood-borne vibrios was studied.At the same time,Psh 10A and Psh 10F were selected as the represent of broad host range to challenge heat inactivation,pH,ultraviolet rays and different magnesium c... |
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| Keywords: | bacteriophage Vibro cholerae Vibrio lysis ability physical and chemical factors |
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