| 应用沉浸式膜生物反应器连续培养虫草 |
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| 引用本文： | 宁尚勇,刘苗苗,许志强,李十中.应用沉浸式膜生物反应器连续培养虫草[J].化工进展,2008,27(8). |
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| 作者姓名： | 宁尚勇 刘苗苗 许志强 李十中 |
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| 作者单位： | 1. 国家副食品质量监督检验中心,北京,100070
2. 清华大学核研究院新能源技术研究所,北京,100084 |
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| 摘 要： | 设计了一种新型沉浸式膜生物反应器(SMBR),并首次将SMBR应用于虫草连续培养过程中。实验表明,间歇发酵7天后转为连续发酵;持续进行6天后,发酵液内菌丝体干重达到33.2g/L,多糖浓度5.4g/L,多糖产率为312mg/(L·h),是间歇发酵的10倍。由此表明,利用本课题组设计的膜生物反应器系统有利于胞外多糖和菌丝体的分离,是生产分离胞外虫草多糖的一条有效途径。
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| 关 键 词： | 沉浸式膜生物反应器 胞外多糖 连续培养 |
Continuous culture of Cordyceps sinensis in submerged membrane bioreactor |
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| NING Shangyong,LIU miaomiao,XU Zhiqiang,LI Shizhong.Continuous culture of Cordyceps sinensis in submerged membrane bioreactor[J].Chemical Industry and Engineering Progress,2008,27(8). |
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| Authors: | NING Shangyong LIU miaomiao XU Zhiqiang LI Shizhong |
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| Affiliation: | NING Shangyong1,LIU miaomiao1,XU Zhiqiang1,LI Shizhong2 |
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| Abstract: | A submerged membrane bioreactor was developed for Cordyceps sinensis fermentation With continuous culture for 6 d,the results showed that the concentration of biomass was 33.2 g/L,the exo-polysaccharide concentration was 5.4 g/L. Moreover,the system was beneficial for Cordyceps sinensis exo-polysacharides (EPS) separation from mycelia in the effluent broth. Also it can be applied to producing EPS of other fungi with high yield. |
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| Keywords: | submerged membrane bioreactor exo-polysaccharides continuous culture |
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