Post-mortem Degradation of Adenine Nucleotides in Muscle of the Lobster, Homarus americanus |
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| Authors: | J R DINGLE J A HINES DORIS I FRASER |
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| Affiliation: | Fisheries Research Board of Canada, Halifax Laboratory, Halifax, N.S. |
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| Abstract: | SUMMARY— Thin-layer chromatography showed that postmortem degradation of adenine nucleotides in the tail muscle of lobster (Homarus americanus) followed the route: adeno-sine 5'-triphosphate (ATP) → adenosine 5'-diphosphate (ADP) → adenosine S'monophosphate (AMP) → inosine 5'-mono-phosphate (IMP) + inosine (Ino) → hypoxanthine (Hx). KCI extracts (0.6M) also degraded ATP by this route. Such extracts contained a weak AMP-aminohydrolase activity that was activated by ATP, but no adenosine aminohydrolase could be detected. Neither of these aminohydrolases were found in extracts made with water or 0.0244 K-succinate. |
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