| 核酸定量技术及其在生物检测中的应用 |
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| 引用本文： | 叶子弘,金荣愉,崔海峰,时鹏涛,俞晓平.核酸定量技术及其在生物检测中的应用[J].中国计量学院学报,2012,23(1):1-6. |
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| 作者姓名： | 叶子弘 金荣愉 崔海峰 时鹏涛 俞晓平 |
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| 作者单位： | 中国计量学院生命科学学院,浙江省生物计量及检验检疫技术重点实验室,浙江杭州310018 |
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| 基金项目： | 国家自然科学基金资助项目(No.31000357);质检公益性行业科研专项(No.201210010);浙江省自然科学基金资助项目(No.Y3090173);杭州市科技攻关项目(No.20101032B28) |
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| 摘 要： | 对目前主要的核酸定量方法(分光光度法、荧光染料法、PCR法、杂交定量法、高效液相色谱法等)及其在生命研究领域中的重大作用进行了述评,并着重阐述了各种核酸定量技术的原理、优缺点,及它们在转基因检测、动植物研究、医药等领域的应用现状.
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| 关 键 词： | 核酸 定量 转基因生物 荧光染料 |
Review on the theory and applications of quantitative techniques of nucleic acid |
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| YE Zi-hong
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JIN Rong-yu
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CUI Hai-feng
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SHI Peng-tao
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YU Xiao-ping.Review on the theory and applications of quantitative techniques of nucleic acid[J].Journal of China Jiliang University,2012,23(1):1-6. |
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| Authors: | YE Zi-hong JIN Rong-yu CUI Hai-feng SHI Peng-tao YU Xiao-ping |
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| Affiliation: | (Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine Technique, College of Life Sciences,China Jiliang University,Hangzhou,310018,China) |
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| Abstract: | The methods and applications of nucleic acids quantification,such as spectrophotometry,fluorescent dye,PCR,hybridization and HPLC were summarized.A brief review of the principles,applications,advantages and disadvantages of these techniques is also given. |
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| Keywords: | nucleic acid quantification GMO fluorescent dye |
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