| 人参稀有皂苷C-K的分离与纯化 |
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| 引用本文： | 郑长龙,鱼红闪,金凤燮. 人参稀有皂苷C-K的分离与纯化[J]. 大连工业大学学报, 2003, 22(3) |
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| 作者姓名： | 郑长龙 鱼红闪 金凤燮 |
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| 作者单位： | 大连轻工业学院,生物与食品工程学院,辽宁,大连,116034;大连轻工业学院,生物与食品工程学院,辽宁,大连,116034;大连轻工业学院,生物与食品工程学院,辽宁,大连,116034 |
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| 摘 要： | 研究了硅胶柱层析法对酶反应产物 皂苷C K粗品进行的分离纯化。当流动相V(氯仿)∶V(甲醇)=9∶1时分离效果最好,可以得到纯度较高的产品。用核磁共振法对产品进行分析,确认产品为人参稀有皂苷C K,即20 O (β D 葡萄糖基) 达玛 24烯,3β,12β,20(S) 三醇。经高效液相色谱进行纯度检测,分离得到的人参皂苷C K的纯度为95%以上,纯品得率为33%,理论得率为60%以上。
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| 关 键 词： | 人参皂苷 柱层析 薄层层析 液相色谱 |
Separating and Purification of Ginsenoside Compund-K |
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| Abstract: | The Ginsenoside Compund K from enzyme reaction was isolated by silica gel column chromatography. The best result can be obtained using the mixture of V(chloroform)∶V(methanol)=9∶1 as mobile phase. The structure is examined by the method of 13C Nuclear Magnetic Resonance (13C NMR) to recognize Compound K, i.e. 20 O (β D glucopyranosyl) dammar 24 en,3β,12β,20(S) triol. The purity of C K is examined by the method of HPLC, it is found that the purity is more than 95%. The C K yield is 33%, and the theoretical yield is over 60%. |
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| Keywords: | Ginsenoside Silica gel column Thin-layer chromatography liquid chromatography |
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