Oxygen consumption,acidification and migration capacity of human primary osteoblasts within a three-dimensional tantalum scaffold |
| |
Authors: | Anika Jonitz Katrin Lochner Tobias Lindner Doris Hansmann Annika Marrot Rainer Bader |
| |
Affiliation: | (1) Department of Orthopaedics, Biomechanics and Implant Technology Research Laboratory, University of Rostock, Doberaner Strasse 142, Rostock, 18057, Germany |
| |
Abstract: | A major clinical problem within synthetic, large-scaled scaffolds is the insufficient nutrient supply resulting in inhomogeneous
cell proliferation and differentiation. The aim of this study was to analyse pH value, oxygen consumption and migration of
human osteoblasts within a 3D tantalum scaffold, clinically used for larger bone defects. After 24 h the oxygen concentration
within the scaffold decreased significantly and remained low during incubation. Monitoring of the pH value inside the tantalum
scaffold showed a slightly acidification under static culture conditions. However, cell migration within the 3D scaffold was
detected. Hence, in clinical application it can be assumed that porous tantalum scaffolds can be settled by osteoblasts under
critical oxygen and nutrient supply. In general, monitoring of cell migration, oxygen consumption and acidification can be
a suitable instrument for creating advanced 3D bone scaffolds. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|