沙门氏菌传统血清凝集分型和分子血清分型试剂盒方法比较

目的 验证沙门氏菌血清分型试剂盒的适用性, 考核本实验室传统沙门氏菌血清分型技术, 扩充沙门氏菌的分型方法, 寻找更有效、更快捷的沙门氏菌分型方法。方法 保存的样品菌株库中随意挑选33株沙门氏菌和6株标准菌株, 首先确证为沙门氏菌, 然后分别采用传统的玻片血清凝集方法和分子血清分型试剂盒对其进行分型, 最后采用16S rRNA系统发育树对试验菌株进行聚类分析。结果 2种分型手段的匹配率高达94.9%, 其中YP 281 Salmonella havana和YP 639 Salmonella liverpool没有匹配到, 这2株菌在试剂盒数据库中不存在, 但本实验利用传统血清分型手段可以对这2株菌进行准确分型, 其中YP 281是本实验室对GB 4789.4-2016《食品安全国家标准 食品微生物学检验 沙门氏菌检验》表B.1外成功分型的沙门氏菌。结论 本实验室传统血清凝集分型技术合格。沙门分子血清分型试剂盒具有较好的适用性, 能更快速、更方便对沙门氏菌进行分型。

Comparison of traditional serum agglutination and molecular serum separation kit for Salmonella

Objective To verify the applicability of Salmonella serotyping kit, examine the traditional Salmonella serotyping technology in our laboratory, expand the methods of Salmonella serotyping, and find more effective and faster methods of Salmonella serotyping. Methods Totally 33 strains of Salmonella and 6 standard strains were randomly selected from the sample library, which were first confirmed as Salmonella, and then classified by traditional slide serum agglutination method and molecular serotyping kit respectively. Finally, the 16S rRNA phylogenetic tree was used for cluster analysis of the test strains. Results The matching rate of the 2 types of methods was as high as 94.9%, and YP 281 Salmonella Havana and YP 639 Salmonella Liverpool did not match. A total of 2 strains did not exist in the kit database, but this experiment could use traditional serotyping methods to accurately classify these 2 strains. Among them, YP 281 was the Salmonella successfully classified by our laboratory outside table B.1 of GB 4789.4-2016 National food safety standard-Food microbiological test-Salmonella test. Conclusion The laboratory''s traditional serum agglutination typing technology is qualified. The Salmonella molecular serotyping kit has good applicability and can be used to classify Salmonella more quickly and conveniently.