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索氏法提取柚籽中柠檬苦素工艺优化研究
引用本文:张早明,李化强,吴菲菲,陈 琼,王恒震. 索氏法提取柚籽中柠檬苦素工艺优化研究[J]. 食品安全质量检测学报, 2019, 10(24): 8331-8336
作者姓名:张早明  李化强  吴菲菲  陈 琼  王恒震
作者单位:邵阳学院食品与化学工程学院,邵阳学院食品与化学工程学院,邵阳学院食品与化学工程学院,邵阳学院食品与化学工程学院,邵阳学院食品与化学工程学院
基金项目:湖南省教育厅科学研究优秀青年项目(17B241), 湖南省自然科学基金青年项目(2017JJ3284), 湖南省科技厅“三区”科技人才项目(S2017NCTPSQ0044), 湖南省教育厅科学研究一般项目(17C1433), 邵阳学院研究生科研创新项目(CX2018SY045)
摘    要:目的 通过响应面优化得到索氏法提取柚籽中柠檬苦素的最佳工艺。方法 样品粉碎过60目筛, 在一定体积的乙醇水溶液中索氏提取一定时间, 高速冷冻离心机离心得到提取液, 稀释、加样、显色, 在491 nm下测定吸光度, 计算安江香柚籽中柠檬苦素的含量。结果 乙醇浓度、提取时间、液料比对安江香柚籽中柠檬苦素的提取均有显著影响; 响应面优化后的提取工艺为: 乙醇浓度90%、提取时间2.2 h、液料比40:1 (mL/g), 得到柠檬苦素提取率最大值11.36 mg/g。结论 利用索氏法提取安江香柚籽中的柠檬苦素方法可行, 效果明显。

关 键 词:安江香柚   柚籽   柠檬苦素   索氏提取   响应面优化
收稿时间:2019-10-22
修稿时间:2019-12-11

Optimization of extraction of limonin from Grapefruit seeds by soxhlet method
ZHANG Zao-Ming,LI Hua-Qiang,WU Fei-Fei,CHEN Qiong and WANG Heng-Zhen. Optimization of extraction of limonin from Grapefruit seeds by soxhlet method[J]. Journal of Food Safety & Quality, 2019, 10(24): 8331-8336
Authors:ZHANG Zao-Ming  LI Hua-Qiang  WU Fei-Fei  CHEN Qiong  WANG Heng-Zhen
Affiliation:Department of Food and Chemical Engineering, Shaoyang University,Department of Food and Chemical Engineering, Shaoyang University,Department of Food and Chemical Engineering, Shaoyang University,Department of Food and Chemical Engineering, Shaoyang University and Department of Food and Chemical Engineering, Shaoyang University
Abstract:Objective To optimize process for extracting limonin from Grapefruit seeds by Soxhlet method by response surface optimization. Methods The sample was pulverized through a 60 mesh sieve, and the mixture is soxed in a certain volume of aqueous ethanol solution for a certain period of time, and the extract is obtained by centrifugation in a high speed refrigerating centrifuge. After dilution, sample loading and color development, the absorbance was measured at 491 nm, and the content of limonin in Anjiangxiang pomelo seeds was calculated. Results Ethanol concentration, extraction time and liquid-to-liquid ratio have significant effects on the extraction of limonin from Anjiangxiang pomelo seeds. The extraction process after optimization of response surface was: ethanol concentration 90%, extraction time 2.2 h, liquid to material ratio 40:1 mL/g, the maximum extraction rate of limonin was 11.36 mg/g. Conclusion The method of extracting limonin from Anjiangxiang pomelo seeds by Soxhlet method is feasible and effective.
Keywords:Anjiang fragrant pomelo   Grapefruit seeds   limonin   Soxhlet extraction   response surface optimization.
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