食品中硒的有机形态分析研究进展

硒是人体必不可少的微量元素, 不同形态的硒对人体的功能不同, 其中有机硒在抗氧化、抗癌、抗病毒、提高免疫力等方面对人体有重要作用。人体获取硒的途径主要通过膳食摄取, 如食用天然富硒食品、人工合成有机硒产品及有机硒强化剂等。市场上的富硒食品质量参差不齐, 为合理地评价食品中硒的摄入形态和水平, 对食品中硒的形态及含量的检测是近年来研究的一个重点。本文对食品中有机硒检测分离、形态分析以及含量测定等进行了综述, 并对以液相萃取法和酶解法为主的分离方法, 以气相色谱-电感耦合等离子体质谱法(gas chromatography-inductively coupled plasma spectrometry, GC-ICP-MS)、高效液相色谱-电感耦合等离子体质谱法(high performance liquid chromatography-inductively coupled plasma spectrometry, HPLC-ICP-MS)、高效液相色谱-串联三重四级杆质谱法(high performance liquid chromatography-tandem mass spectrometry, HPLC-MS/MS)以及电泳-电感耦合等离子体质谱法(capillary electrophoresis-inductively coupled plasma spectrometry, CE-ICP-MS)为主的检测方法等研究进展进行了总结, 以期对食品中有机硒的检测提供相应的理论依据, 促进食品中有机硒检测标准的发展。

Research progress of organic selenium speciation analysis in food

Selenium is an indispensable trace element in the human body, which has various functions due to the different forms. Organic selenium plays an important role in the body while is anti-oxidation, anti-cancer, anti-virus, and improving immunity. The main way for human body to obtain selenium is dietary intake, such as natural selenium rich foods, synthetic organic selenium products and organic selenium fortifiers. The uneven qualities for the food products of selenium-rich on the market have been a problem to assess the reasonable level and effective of selenium in food intake, while the forms inspection and content assay of selenium in food is one of the stressed research areas for food tests in recent years. This paper summarized the separation of organic selenium in food detection, morphological analysis, and content determination, including the research progress of the separation methods of liquid phase extraction and enzymatic hydrolysis, as well as gas chromatography-inductively coupled plasma mass spectrometry (GC-ICP-MS), high performance liquid chromatography with inductively coupled plasma mass spectrometry (HPLC-ICP-MS), high performance liquid chromatography-tandem mass spectrometry (HPLC/MS/MS), and electrophoresis-inductively coupled plasma mass spectrometry (CE-ICP/MS) based detection methods, in order to provide the corresponding theoretical basis for tests of organic selenium in food, which also promoting the development of organic selenium inspection methods.