Rapid transmethylation of microgram amounts of phosphatidylcholine on potassium methoxide/celite columns1,2,3 |
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Authors: | William N Marmer |
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Affiliation: | (1) Eastern Regional Research Center, Federal Research, Science and Education Administration, U.S. Department of Agriculture, 19118 Philadelphia, PA |
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Abstract: | A rapid and convenient method for the determination of acyl groups in phosphatidylcholine (PC) has been developed. Transmethylation
reactions were carried out on potassium methoxideimpregnated Celite microcolumns that were readily prepared from Pasteur pipettes.
The methods were tested on microgram amounts of synthetic L-α-phosphatidylcholines (di-14∶0; di-18∶2; α-16∶0-β-14∶0) and on
egg yolk lecithin, in methylene chloride or hexane solution. Transmethylation of these lipids occurred rapidly at room temperature.
Gas liquid chromatographic (GLC) analysis of the product methyl esters demonstrated that the reaction was neither selective
for one acyl position of PC over the other, nor sensitive to the amount of unsaturation within the acyl group. The results
of the acyl group analysis of natural egg yolk lecithin compared favorably with the results from an established procedure.
Presented at AOCS 68th Annual Meeting, New York, May 1977.
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Chloroform and carbon disulfide have been classified by OSHA as carcinogens and/or toxic materials; they should be handled
with care under proper ventilation. |
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