细毛石花菜多糖自由基清除能力和理化性质的研究

该文通过研究细毛石花菜(Gelidium crinale)多糖提取物对自由基的清除能力及理化性质,为细毛石花菜的高值化加工利用提供实验依据.以细毛石花菜为原料,用0.1 mol/L的HCl提取制备细毛石花菜多糖,并对其理化性质和抗氧化活性进行研究.结果显示,细毛石花菜多糖中总糖占比为70.51％,还原糖占比为8.42％...

Research on the free radical scavenging ability and physicochemical properties of the polysaccharides in Gelidium crinale

Gelidium crinaleis a marine economic red algae belonging to the Gelidium.By studying the scavenging ability and physical and chemical properties of its polysaccharides extracts on free radicals,it provides an experimental basis for the high-value processing and utilization of Gelidium crinale.In this study,Gelidium crinale was used as raw material and polysaccharides were extracted from Gelidium crinale with 0.1 mol/L HCl.Its physical and chemical properties and antioxidant activity were studied.The results showed that the proportion of total sugars and reducing sugar in polysaccharides were 70.51%and 8.42%,respectively.Moreover,the proportion of sulfate group and protein were 16.50%and 5.27%.Analysis of monosaccharide composition showed that the top three were galactose,fucose and xylose which with a relative molecular weight of 25.8 kDa.The results of in vitro antioxidant results showed that polysaccharides from Gelidium crinale had good antioxidant activity.The IC50 of its ABTS free radical scavenging ability,Fe²⁺ chelating ability,DPPH free radical scavenging ability and hydroxyl free radical scavenging ability were 2.215,2.688,7.408,13.56 mg/mL,respectively.The results of cell experiments showed that the level of superoxide dismutase(SOD)in cells was significantly increased in a dose-dependent manner after treatment with polysaccharide from Gelidium crinale,but it inhibited the production of reactive oxygen species(ROS)(P<0.001).