酯化微孔玉米淀粉制备及其吸附番茄红素的研究

为了提高番茄红素的储存稳定性,首先以玉米淀粉为原料,辛烯基琥珀酸酐为酯化剂,α-淀粉酶为酶解剂,制备了酯化微孔淀粉,测定了酯化微孔淀粉的取代度及水解度,并通过红外光谱及扫描电镜对其进行了表征。其次将其应用于吸附番茄红素,以番茄红素吸附量为指标,考察了吸附浓度、吸附时间、吸附温度的影响,并测定了酯化微孔淀粉吸附番茄红素的饱和吸附量。最后测定了酯化微孔淀粉吸附番茄红素在自然光、紫外光、不同温度及氧气存在不同条件下的稳定性。结果表明,制备的酯化微孔淀粉取代度DS=0.0227,水解度DE=0.46,且经红外光谱及扫描电镜表征说明制备的酯化微孔淀粉不仅成功引入了酯基且具有良好的开孔性能。单因素实验表明较佳吸附浓度、吸附时间、吸附温度分别为10μg/m L,30 min,45℃。酯化微孔淀粉对番茄红素饱和吸附量高达225.45μg/g。稳定性实验表明,酯化微孔淀粉吸附的番茄红素具有良好的稳定性,因此酯化微孔淀粉可用于番茄红素的保存。 

Preparation of esterified microporous corn starch and its adsorption property of lycopene

In order to improve the storage stability of lycopene,firstly corn starch as raw material,octenyl succinate anhydride as esterifying agent and α-amylase as enzymatic hydrolysis agent to prepare esterified microporous starch. The esterified microporous starch of degrees of substitution and degree of hydrolysis was determined.The characterization of esterified microporous starch was carried out by FT-IR spectra and scanning electron microscopy. The influence of the adsorption time,adsorption temperature and adsorption concentration on the esterified microporous starch adsorption of lycopene were studied. The stability of adsorbed lycopene by esterified microporous starch under natural light,ultraviolet light,differing temperatures and oxygen was tested.The esterified microporous starch with substitution degree 0.0227 and degree of hydrolysis 0.46 was characterized by FT-IR spectra and scanning electron microscopy,the results showed the ester group was introduced and had a good opening performance. The single factor test showed that the better adsorption concentration,adsorption time,adsorption temperature were 10 μg/m L,30 min,45 ℃. The saturated adsorption amount of lycopene by esterified microporous starch was 225.45 μg/g. Compared with native starch,the adsorption ability and adsorption stability of the esterified microporous starch to lycopene had improved significantly,and the adsorption stability was also improved. So the esterified microporous starch could be used for the preservation of the lycopene.