超高效液相色谱串联质谱法检测婴儿配方奶粉中的双氰胺

目的：建立一种婴儿配方奶粉中双氰胺残留量的超高效液相色谱质谱检测方法。方法：采用三氯乙酸溶液沉淀蛋白，处理液用watersSep—PakAc2固相萃取柱净化，用超高效液相色谱一串联质谱仪测定，外标法定量。结果：在0．10I．Lg／mL-50．0μg／mL范围内，线性良好，线性方程为y=2310．43×x＋1．01815，相关系数为0．999826（n=6）。加标回收率在90％～110％，相对标准偏差在10％1）2内。最低检出限为0．05mg／kg。结论：本方法操作简便，检出限低，重复性好，适合婴儿配方奶粉中双氰胺残留的测定。

Determination of Dicyandiamide residue in Infant Formula Milk Powder by Ultra Performance Liquid Chromatography Combined with Mass Spectrometry

[Objective] A new method to detect the dicyandiamide residue in infant formula milk powder by using ultra high performance liquid chromatography combined with mass spectrometry is established. [ Methods ] After protein precipitation with trichloroacetic acid solution, the treatment fluid was purified by using a Waters Sep-Pak AC2 solid phase extraction column, and then determinated by ultra-high performance liquid chromatography combined with mass spectrometry, and external standard method was used for quantification. [ Results ] In the range of 0.10 μg/mL - 50.0 μg/mL range, the results showen good linearity, the linear equation is y=2310.43x＋l.01815, and the correlation coefficient is 0.999826 （ n=6 ） . The recovery is 90% - 110%, the relative standard deviation is less than 10%, and the detection limit is 0.05 mg/kg. [Conclusions] This method is simple, with low detection limit and good repeatability to detect the dicyandiamide residue in infant formula milk powder.