碳源和进水pH值对聚糖菌代谢的短期影响

通过连续流A/O工艺驯化富集了聚糖菌(GAOs),在此基础上利用静态批次试验分别考察了碳源(乙酸、丙酸和葡萄糖)和进水pH值(6.5、7.0和7.5)对GAOs代谢的短期冲击影响。结果表明,在厌氧阶段,GAOs污泥可以较快的速率吸尽乙酸和丙酸,而葡萄糖仅部分被吸收。对比消耗单位碳源合成的聚-β-羟基烷酸(PHA)量,以葡萄糖为碳源时最大,以丙酸作为碳源时最小。同时,GAOs污泥吸收单位碳源所消耗的糖原量以丙酸最少;而以葡萄糖为碳源时,与乙酸和丙酸情况不同的是其在厌氧反应后期糖原量略有增加。另外,在考察进水pH值对GAOs污泥代谢的短期冲击影响时发现,随着进水pH值的升高,GAOs污泥对挥发性脂肪酸(VFAs)的吸收速率减缓,但进水pH值的短期波动对于胞内物质(糖原、PHA)代谢的影响不大。

Short-term Effect of Carbon Sources and Influent pH on Metabolism of Glycogen Accumulating Organisms

Batch experiments were conducted to investigate the short-term effect of different carbon sources(acetic acid,propionic acid and glucose) and influent pH(6.5,7.0 and 7.5) on the metabolism of glycogen accumulating organisms(GAOs),which were acclimatized in a continuous flow anaerobic/aerobic(A/O) process.The results show that the GAOs sludge can quickly take up propionic acid and acetic acid,while glucose is not consumed completely.When comparing the poly-β-hydroxyalkanoates(PHA) synthesized per unit carbon source consumed,the synthesis amount is maximum when using glucose as the carbon source,and this synthesis amount are minimum when using propionic acid as the carbon source.However,in the case of glucose,glycogen is increased in the later period of anaerobic phase as compared with that of acetic acid and propionic acid.The pH experiments show that as the influent pH is increased,VFAs uptake rate is decreased;however,the short-term change of influent pH has no great impact on the metabolism of intracellular substance such as glycogen and PHA.