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Biobased Agents for Single-Particle Detection with Optoacoustics (Small 29/2023) |
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| Authors: | Yunbo Chen Daniil Nozdriukhin Sandra Michel-Souzy Clemens Padberg Frederik R. Wurm Daniel Razansky Xosé Luís Deán-Ben Olga Koshkina |
| Affiliation: | 1. Sustainable Polymer Chemistry, Department of Molecules and Materials, Mesa+ Institute for Nanotechnology, Faculty of Science and Technology, University of Twente, Drienerlolaan 5, Enschede, 7522NB The Netherlands Key Lab of Science and Technology of Eco-Textile, Ministry of Education, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, 201620 P. R. China;2. Institute for Biomedical Engineering and Institute of Pharmacology and Toxicology, Faculty of Medicine, University of Zürich, Winterturenstraße 190, Zürich, 8057 Switzerland Institute for Biomedical Engineering, Department of Information Technology and Electrical Engineering, ETH Zürich, Wolfgang-Pauli-Str. 27, Zürich, 8093 Switzerland;3. Biomolecular Nanotechnology, Department of Molecules and Materials, Mesa+ Institute for Nanotechnology, Faculty of Science and Technology University of Twente, Drienerlolaan 5, Enschede, 7522NB The Netherlands;4. Sustainable Polymer Chemistry, Department of Molecules and Materials, Mesa+ Institute for Nanotechnology, Faculty of Science and Technology, University of Twente, Drienerlolaan 5, Enschede, 7522NB The Netherlands |
| Abstract: | Optoacoustic (OA, photoacoustic) imaging synergistically combines rich optical contrast with the resolution of ultrasound within light-scattering biological tissues. Contrast agents have become essential to boost deep-tissue OA sensitivity and fully exploit the capabilities of state-of-the-art OA imaging systems, thus facilitating the clinical translation of this modality. Inorganic particles with sizes of several microns can also be individually localized and tracked, thus enabling new applications in drug delivery, microrobotics, or super-resolution imaging. However, significant concerns have been raised regarding the low bio-degradability and potential toxic effects of inorganic particles. Bio-based, biodegradable nano- and microcapsules consisting of an aqueous core with clinically-approved indocyanine green (ICG) and a cross-linked casein shell obtained in an inverse emulsion approach are introduced. The feasibility to provide contrast-enhanced in vivo OA imaging with nanocapsules as well as localizing and tracking individual larger microcapsules of 4–5 µm is demonstrated. All components of the developed capsules are safe for human use and the inverse emulsion approach is known to be compatible with a variety of shell materials and payloads. Hence, the enhanced OA imaging performance can be exploited in multiple biomedical studies and can open a route to clinical approval of agents detectable at a single-particle level. |
| Keywords: | biobased emulsion polymerization nanocarriers optoacoustics proteins |