History of genome editing in yeast |
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Authors: | Marcin G Fraczek Samina Naseeb Daniela Delneri |
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Affiliation: | The University of Manchester, Faculty of Biology, Medicine and Health, Manchester Institute of Biotechnology, Manchester, UK |
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Abstract: | For thousands of years humans have used the budding yeast Saccharomyces cerevisiae for the production of bread and alcohol; however, in the last 30–40 years our understanding of the yeast biology has dramatically increased, enabling us to modify its genome. Although S. cerevisiae has been the main focus of many research groups, other non‐conventional yeasts have also been studied and exploited for biotechnological purposes. Our experiments and knowledge have evolved from recombination to high‐throughput PCR‐based transformations to highly accurate CRISPR methods in order to alter yeast traits for either research or industrial purposes. Since the release of the genome sequence of S. cerevisiae in 1996, the precise and targeted genome editing has increased significantly. In this ‘Budding topic’ we discuss the significant developments of genome editing in yeast, mainly focusing on Cre‐loxP mediated recombination, delitto perfetto and CRISPR/Cas. |
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Keywords: | Cre‐loxP CRISPR/Cas9 delitto perfetto genome editing yeast |
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