| 反相高效液相色谱法检测酵母胞内V_B_1含量 |
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| 引用本文： | 肖冬光,毕琳,韩英素. 反相高效液相色谱法检测酵母胞内V_B_1含量[J]. 食品与发酵工业, 2005, 31(10): 120-122 |
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| 作者姓名： | 肖冬光 毕琳 韩英素 |
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| 作者单位： | 天津科技大学生物工程学院,天津,300222 |
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| 摘 要： | 采用反相高效液相色谱法测定了酵母胞内VB1的含量。采用ODS2HYPERSIL色谱柱(5μm,250×4.6mm),优化后的色谱条件为:流动相V(甲醇)∶V(水)=3∶7,流速1.2mL/min,波长238nm,柱温35℃。在此条件下回收率为88%～92%。此方法步骤简单,重现性好。
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| 关 键 词： | 酵母 VB1 反相高效液相色谱法(RPHPLC) |
| 收稿时间： | 2005-07-13 |
| 修稿时间： | 2005-08-29 |
Determination of Intracellular Thiamine of Yeast by RP-HPLC |
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| Xiao Dongguang,Bi Lin,Han Yingsu. Determination of Intracellular Thiamine of Yeast by RP-HPLC[J]. Food and Fermentation Industries, 2005, 31(10): 120-122 |
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| Authors: | Xiao Dongguang Bi Lin Han Yingsu |
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| Abstract: | A quantitative method was developed for the determination of Vitamin B_1 in the yeast cells by RP-HPLC.The chromatographic column was ODS-2 HYPERSIL(5 μm, 250 mm×4.6 mm).The optimal chromatographic conditions including methanol and water at 3∶7 were served as mobile phase, and flow rate was 1.2 mL/min at 238 nm by using UV-detector; column temperature was 35℃. The methods recovery rate was 88%～92%. The method is simple and reproducible. |
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| Keywords: | yeast thiamine RP-HPLC |
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