Sialyloligosaccharides of Delipidated Egg Yolk Fraction |
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| Authors: | MAMORU KOKETSU LEKH RAJ JUNEJA MUJO KIM MASAYA OHTA FUMITO MATSUURA TAKEHIKO YAMAMOTO |
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| Affiliation: | Authors Koketsu, Juneja, and Kim are with Central Research Laboratories, Taiyo Kagaku Co., Ltd., 1–3 Takaramachi, Yokkai-chi, Mie 510, Japan. Authors Ohta, Matsuura, and Yamamoto are with the Dept. of Biotechnology, Faculty of Engineering, Fukuyama University, Fukuyama, Hiroshima 729–02, Japan. |
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| Abstract: | Delipidated egg yolk (DEY) was homogenized and centrifuged. The supernatant was dialyzed by ultrafiltration using a molecular weight cut-off of 1,000. The asparagine-linked oligosaccharides in the concentrate were liberated from protein by hydrazinolysis and labeled with UV-absorbingp-amionbenzoic ethyl ester (ABEE). The ABEE-derivatized oligosaccharides were fractionated by anion exchange and reverse-phase HPLC. The total sialyloligosaccharides from the water-soluble DEY fraction, were about 47.7% monosialyl and 50.6% di-sialyloligosaccharides. The structures of 3 predominant sialyloligosaccharides were determined by NMR. They were of the biantennary complex-type, accounting for about 33.6% of the total sialyloligosaccharides of the DEY water-soluble fraction. |
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| Keywords: | egg yolks sialyloligosaccharides sialic acid |
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