Enzymatic fractionation of fatty acids: Enrichment of γ-linolenic acid and docosahexaenoic acid by selective esterification catalyzed by lipases |
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Authors: | Matthew J Hills Irmgard Kiewitt Kumar D Mukherjee |
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Affiliation: | (1) Federal Center for Lipid Research, Institute for Biochemistry and Technology, H.P. Kaufmann-Institute, Piusallee 68, D-4400 Münster, Feaoral Republic of Germany |
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Abstract: | Immobilized lipase preparations from seedlings of rape (Brassica napus L.) andMucor miehei (lipozyme) used as biocatalysts in esterification and hydrolysis reactions discriminate strongly against γ-linolenic and
docosahexaenoic acids/acyl moieties. Utilizing this property, γ-linolenic acid contained in fatty acids of evening primrose
oil has been enriched seven to nine-fold, from 9.5 to almost 85% by selective esterification of the other fatty acids with
butanol. Similarly, docosahexaenoic acid of cod liver oil has been enriched four to five-fold, from 9.4 to 45% by selective
esterification of fatty acids (other than docosahexaenoic acid) with butanol. As long as the reaction is stopped before reaching
equilibrium, very little of either γ-linolenic acid or docosahexaenoic acid are converted to butyl esters, which results in
high yields of these acids in the unesterified fatty acid fraction. |
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Keywords: | Cod liver oil docosahexaenoic acid enzymatic fractionation of fatty acids evening primrose oil γ -linolenic acid lipase-catalyzed esterification Mucor miehei lipase rape lipase |
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