| 乳糖发酵短杆菌lysC突变对L-赖氨酸积累的影响 |
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| 引用本文： | 董迅衍,徐大庆,李烨,李江华,王小元. 乳糖发酵短杆菌lysC突变对L-赖氨酸积累的影响[J]. 食品与生物技术学报, 2011, 30(4): 592-596 |
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| 作者姓名： | 董迅衍 徐大庆 李烨 李江华 王小元 |
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| 作者单位： | 1. 食品科学与技术国家重点实验室,江南大学,江苏无锡214122
2. 江南大学生物工程学院江苏无锡214122 |
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| 基金项目： | 国家863计划项目(2007AA02Z229和2007AA02Z230) |
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| 摘 要： | 从一株乳糖发酵短杆菌L-异亮氨酸生产菌中克隆出天冬氨酸激酶AK-1的编码基因ly-sC1,经DNA测序并与来自谷氨酸棒杆菌ATCC13032的野生型lysC比对发现其中有2个核苷酸1186G和1187C缺失,造成翻译提前终止。AK-1还有下列2个有效突变位点:Ala 279 Thr和Ser317 Ala。lysC1在大肠杆菌BL21中的诱导表达量约为lysC的1/4,其表观比酶活也较低,但对L-苏氨酸和L-赖氨酸协同反馈抑制不敏感。用大肠杆菌-黄色短杆菌穿梭表达载体pDXW-8将ly-sC1在野生型乳糖发酵短杆菌ATCC13869中诱导型表达,经摇瓶发酵积累L-赖氨酸7.4 g/L,在3 L发酵罐上达40 g/L。
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| 关 键 词： | 乳糖发酵短杆菌 天冬氨酸激酶 抗反馈抑制 发酵 L-赖氨酸 |
The Effect of LysC on L-Lysine Accumulation in Brevibacterium lactofermentum |
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| DONG Xun-yan,XU Da-qing,LI Ye,LI Jiang-hua and WANG Xiao-yuan. The Effect of LysC on L-Lysine Accumulation in Brevibacterium lactofermentum[J]. Journal of Food Science and Biotechnology, 2011, 30(4): 592-596 |
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| Authors: | DONG Xun-yan XU Da-qing LI Ye LI Jiang-hua WANG Xiao-yuan |
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| Affiliation: | DONG Xun-yan1,XU Da-qing1,LI Ye1,LI Jiang-hua2,WANG Xiao-yuan1(1.State Key Laboratory of Food Science and Technology,Jiangnan University,Wuxi 214122,China,2.School of Biotechnology,China) |
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| Abstract: | The gene lysC1 encoding aspartate kinase AK-1 from a Brevibacterium lactofermentum L-isoleucine producer was sequenced.Compared with the lysC from Corynebacterium glutamicum ATCC13032,there were deletions of two nucleotides 1186G and1187C,leading to the pre-termination of translation.In addition,the following two mutated sites were also identified in lysC1: Ala 279 Thr and Ser 317 Ala.In Escherichia coli BL21,the expression level of lysC1 was lower than that of lysC from B.lactofermentum ATCC13869.The AK-1 ... |
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| Keywords: | Brevibacterium lactofermentum aspartate kinase feedback inhibition fermentation L-lysine |
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