Detection of chlorophyll derivatives in soybean oil by HPLC |
| |
Authors: | M. S. Fraser G. Frankl |
| |
Affiliation: | (1) Technical Center, Hunt-Wesson Foods, Inc., 1645 W. Valencia Dr., 92634 Fullerton, CA |
| |
Abstract: | Chlorophyll derivatives have been isolated from a degummed soybean oil by cellulose column chromatography and resolved by reversed phase HPLC. The HPLC separation was performed on a Zorbax ODS column using acetone-methanol (75:25) as the mobile phase. Seven major components were detected by visible (650 nm) light absorption. Pheophytin A is the predominant component of the mixture (40–45% of the total). Pheophytin A’, pyropheophytin A, and three unidentified pigments having spectral features resembling pheophytin A comprise the other major pigments. No evidence was found for the presence of chlorophylls A and B in this oil. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|