UPLC法同时检测新疆葡萄酒中的7种单体酚

建立了一种测定葡萄酒中7种单体酚含量的超高效液相色谱（UPLC）检测方法。采用ACQUITY BEH C18色谱柱，以甲醇/乙酸铵-乙酸缓冲溶液为流动相，0.2 mL/min梯度洗脱，变波长检测，各化合物线性关系较好（R2＞0.993），不同的添加水平的回收率在84.0%~108.4%范围，方法重复性较好（RSD≤6.08%）。该文利用UPLC法分析了不同地区、不同年份的葡萄酒中7种单体酚的含量，结果表明，葡萄酒中含有丰富的单体酚，单体酚的含量因酒样含糖量、品牌、厂家和原料产地不同而差别很大。

Determination of 7 mono-phenols in Xinjiang wines by UPLC

A UPLC method was developed for determining 7 mono-phenols in wines. The chromatographic separation was performed on an ACQUITY BEH C18 column eluted with mobile phase of methanol/ammonium acetate-acetic acid mixture at a flow rate of 0.2 ml/min. The detection wavelength was variable with the change of maximum wavelength. The calibration curves of 7 mono-phenols were linear with correlation coefficients more than 0.993, recovery rate 84.0%-108.4%, and the method repeatability was good (RSD≤6.08%). Different aging times and different geographical origins of wine were determined. The results showed that wine was rich in mono-phenols, and their contents were distinct with the difference of sugar content, brand, producer and geographical origins of raw materials.