Isolation and characterization of the monounsaturated long chain fatty acids ofMycobacterium tuberculosis |
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| Authors: | Kuni Takayama Nilofer Quereshi Heinrich K Schnoes |
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| Affiliation: | (1) Tuberculosis Research Laboratory, Williams S. Middleton Memorial Veterans Administration Hospital, 53706 Madison, Wilsconsin;(2) The Institute for Enzyme Research, University of Wisconsin, 53706 Madison, Wilsconsin;(3) Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, 53706 Madison, Wisconsin |
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| Abstract: | The positions of double bond in the monounsaturated C15−C32 fatty acids ofMycobacterium tuberculosis H37Ra were established by gas chromatography/mass spectrometry of the ozonized esters and their pyrrolidide derivatives.
The monounsaturated C15−C21 fatty acids had the double bond primarily at the Δ9 position while the monounsaturated longer chain fatty acids (C22−C32) had the double bond in several positions. Many of the latter acids, especially the odd-numbered series, were very complex
isomeric mixtures. Quantitation showed the most abundant even-numbered long chain fatty acid isomers to be as follow: C22, Δ4; C24, Δ5; C26, Δ7 and Δ9; C28, Δ9; C30, Δ11 and Δ13; C32, Δ13 and Δ15. |
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