| ISEC法在评价色谱介质孔径结构方面的应用 |
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| 引用本文： | 池伟亚,侯恒磊,康红艳,齐莉,靳海波,张荣月. ISEC法在评价色谱介质孔径结构方面的应用[J]. 精细化工, 2020, 37(6) |
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| 作者姓名： | 池伟亚 侯恒磊 康红艳 齐莉 靳海波 张荣月 |
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| 作者单位： | 北京石油化工学院,北京石油化工学院,唐山第八医院检验科,中国科学院化学研究所,北京石油化工学院,北京石油化工学院 |
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| 基金项目： | 北京市委组织部青年拔尖人才项目(ZZB2019001)；北京市属高校高水平教师队伍建设支持计划高水平创新团队建设计划项目（IDHT20180508） |
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| 摘 要： | 层析是生物大分子分离纯化的一种重要手段，层析介质作为层析过程的关键的一部分受到了广泛的关注。层析介质的孔径结构对其分离纯化的效果有着显著的影响，因此探究层析介质的孔径结构至关重要。介绍了测定色谱介质孔径结构常用的方法，如：压汞法、氮气吸附法、小角X射线散射法、电镜扫描法（SEM和TEM）和逆体积排阻色谱法(ISEC)。重点阐述了ISEC法的基本原理和常用的探针类型，总结了ISEC法应用中的优点及不足，介绍了ISEC法的发展历史及其在国内外的研究现状。
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| 关 键 词： | 逆体积排阻色谱法；层析介质；孔径结构；测定方法 |
| 收稿时间： | 2020-01-09 |
| 修稿时间： | 2020-03-15 |
Recent progress in application of ISEC in evaluating the pore size structure of chromatographic medias |
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| chiweiy,houhenglei,KANGHongyan,qili,jinhaibo and ZHANG Rong-yue. Recent progress in application of ISEC in evaluating the pore size structure of chromatographic medias[J]. Fine Chemicals, 2020, 37(6) |
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| Authors: | chiweiy houhenglei KANGHongyan qili jinhaibo ZHANG Rong-yue |
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| Affiliation: | Beijing Institute of Petrochemical Technology,Beijing Institute of Petrochemical Technology,Tangshan Eighth hospital,Institute of Chemistry Chinese Academy of Sciences,Beijing Institute of Petrochemical Technology,Beijing Institute of Petrochemical Technology |
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| Abstract: | Chromatography is one of the methods for separation and purification of biomolecules, and chromatography media has been widely concerned as a key part of the chromatography process. The pore structure of the chromatographic medium has a significant influence on its separation and purification efficiency. Therefore, it is very important to explore the pore structure of the chromatographic medium. This paper introduced several methods commonly used to determine the pore diameter structure of chromatographic media, such as mercury intrusion porosimetry, nitrogen adsorption, small angle X-ray scattering, transmission electron microscopy (TEM), scanning electron microscopy (SEM) and inverse size-exclusion chromatography (ISEC). The basic principle and common probe types of ISEC are described, the advantages and disadvantages in the application of ISEC are summarized, the development history of ISEC and its research advance are introduced in this paper. |
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| Keywords: | inverse size-exclusion chromatography chromatography media pore structure determination method |
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