| 超声波辅助法提取细叶黄皮叶总黄酮及其清除羟自由基活性的测定 |
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| 引用本文： | 苏秀芳,郭青,甘海妹,黄智想.超声波辅助法提取细叶黄皮叶总黄酮及其清除羟自由基活性的测定[J].化学世界,2011,52(7):420-422,431. |
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| 作者姓名： | 苏秀芳 郭青 甘海妹 黄智想 |
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| 作者单位： | 1. 广西民族师范学院化学与生物工程系,广西崇左,532200
2. 江苏省食品药品检验所,江苏南京,210008 |
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| 基金项目： | 广西自然科学基金项目(2010GXNSFA013050) |
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| 摘 要： | 对超声波法提取细叶黄皮叶中黄酮类物质进行了研究,通过单因素及L9(3(4))正交实验,最佳提取工艺条件为A1B3C3D3,即:温度60℃,乙醇浓度60%,超声波功率90 W,料液比1:25,提取时间70 min.此条件下细叶黄皮叶总黄酮提取率达5.730%.在设置的5个质量浓度梯度下,总黄酮提取液对羟自由基的清除效果与...
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| 关 键 词： | 细叶黄皮叶 总黄酮 超声波辅助提取 羟自由基 |
Ultrasonic-assisted Extraction Technology and Hydroxy Radical Scavenging Activity of Total Flavonoids from the Leaves of Clausena Anisum-olens |
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| SU Xiu-fang,GUO Qing,GAN Hai-mei,HUANG Zhi-xiang.Ultrasonic-assisted Extraction Technology and Hydroxy Radical Scavenging Activity of Total Flavonoids from the Leaves of Clausena Anisum-olens[J].Chemical World,2011,52(7):420-422,431. |
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| Authors: | SU Xiu-fang GUO Qing GAN Hai-mei HUANG Zhi-xiang |
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| Affiliation: | SU Xiu-fang1,GUO Qing2,GAN Hai-mei1,HUANG Zhi-xiang1 (1.Department of Chemistry and Biological Engineering,Guangxi Normal Universty for Nationalities,Guangxi,Chongzuo 532200,China,2.Jiangsu Institute for Food and Drug Control,Jiangsu Nanjing 210008,China) |
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| Abstract: | The ultrasonic-assisted extraction of flavonoids in the leaves of Clausena anisum-olens was studied.The single factor and orthogonal design methods L9(34) were applied for obtaining the optimal technological conditions:temperature at 60 ℃,60 % ethanol,ratio of material to liquid=1∶25,power of 90 W,and extraction time of 70 min.Under the optimal conditions,the extraction percent of total flavonoids was about 5.730 % from the leaves of Clausena anisum-olens.There was a positive correlation between hydroxyl radical scavenging activity and concentration of total flavonoids from the leaves of Clausena anisum-olens.The hydroxyl radical scavenging rate at the concentration level of 0.649 g/L was 49.1%. |
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| Keywords: | leaves of Clausena anisum-olens total flavonoids ultrasonic-assisted extraction hydroxy free radical |
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