| 氰戊菊酯残留胶体金免疫层析试纸条研制 |
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| 引用本文： | 肖琛,李培武,唐章林,张奇,张文.氰戊菊酯残留胶体金免疫层析试纸条研制[J].化学试剂,2011(8):675-679. |
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| 作者姓名： | 肖琛 李培武 唐章林 张奇 张文 |
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| 作者单位： | 西南大学农学与生物科技学院;中国农业科学院油料作物研究所;农业部油料作物生物学重点开放实验室;农业部油料及制品质量监督检验测试中心; |
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| 基金项目： | 国家自然科学基金资助项目(30800771); 武汉市晨光青年计划资助项目(200950431224); 武汉市科技研发中心平台项目(200820337086); 国家油菜产业体系(nycytx-005) |
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| 摘 要： | 应用胶体金免疫层析技术研制出一种准确、快速、简便检测氰戊菊酯农药残留的试纸条.采用柠檬酸三钠还原法制备胶体金,标记氰戊菊酯单克隆抗体,以硝酸纤维素膜为载体,包被氰戊菊酯抗原作为检测线(T线),包被兔抗鼠IgG抗体作为质控线(C线),经过竞争反应后目测结果.实验结果表明,该快速检测试纸条100%抑制浓度为800 ng/m...
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| 关 键 词： | 氰戊菊酯 胶体金 免疫层析试纸条 快速检测 |
Development of gold immunochromatography strip for fenvalerate residue |
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| XIAO Chen,LI Pei-wu,,TANG Zhang-lin,ZHANG Qi,ZHANG Wen.Development of gold immunochromatography strip for fenvalerate residue[J].Chemical Reagents,2011(8):675-679. |
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| Authors: | XIAO Chen LI Pei-wu TANG Zhang-lin ZHANG Qi ZHANG Wen |
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| Affiliation: | XIAO Chen1,LI Pei-wu2,3,4,TANG Zhang-lin1,ZHANG Qi2,ZHANG Wen2,4(1.College of Agronomy and Biotechnology,Southwest University,Chongqing 400716,2.Oil Crops Research Institute of Chinese Academy of Agriculture Science,Wuhan 430062,3.Key Laboratory of Oil Crops Biology of Ministry of Agriculture,4.Quality Inspection and Test Center for Oilseeds Product,MOA,China) |
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| Abstract: | A method for highly selective fenvalerate detection was reported,which based on colloidal gold probes.Immunochromatography assay based on monoclonal antibody(McAb) colloidal gold labeling technique was developed for the fenvalerate based on competitive inhibition principle.McAb against fenvalerate was conjugated to colloidal gold which synthesized by reducing reaction of sodium citrate.Conjugated fenvalerate-BSA acting as the coating antigen and rabbit anti-mouse IgG were immobilized on the nitrocellulose m... |
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| Keywords: | fenvalerate colloidal gold immunochromatographic(IC) strip rapid detection |
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