| 脂质体催化其表面甘氨酸与磷酸吡哆醛形成希夫碱 |
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| 引用本文： | 刘宝全,王剑锋,李春斌,范圣第.脂质体催化其表面甘氨酸与磷酸吡哆醛形成希夫碱[J].化学世界,2011,52(8):497-499,503. |
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| 作者姓名： | 刘宝全 王剑锋 李春斌 范圣第 |
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| 作者单位： | 大连民族学院生命科学学院生物技术与资源利用国家民委-教育部重点实验室,辽宁大连,116600 |
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| 基金项目： | 国家自然科学基金资助项目(20872013); 教育部科学技术研究重点项目(2010); 国家民委基金(09DL08); 辽宁省教育厅项目(2009A154) |
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| 摘 要： | 利用脂质体的疏水微环境,完成了甘氨酸与磷酸吡哆醛的希夫碱反应.甘氨酸进行双烷基化修饰后,组装到脂质体中.利用肽脂质构建带正电荷的脂质体(pH=7.00),吸附带负电荷的磷酸吡哆醛,增强磷酸吡哆醛与脂质体上甘氨酸的伯胺基团作用,并通过脂质体疏水微环境促进脱水缩合过程.紫外光谱检测结果表明,单独的磷酸吡哆醛吸收峰为387 ...
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| 关 键 词： | 希夫碱 脂质体 甘氨酸 磷酸吡哆醛 铜离子 |
The Formation of Schiff Base between Modified Glycine and PLP on Liposome Surface |
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| LIU Bao-quan,WANG Jian-feng,LI Chun-bin,FAN Sheng-di.The Formation of Schiff Base between Modified Glycine and PLP on Liposome Surface[J].Chemical World,2011,52(8):497-499,503. |
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| Authors: | LIU Bao-quan WANG Jian-feng LI Chun-bin FAN Sheng-di |
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| Affiliation: | LIU Bao-quan,WANG Jian-feng,LI Chun-bin,FAN Sheng-di(Key Laboratory of Biotechnology & Bioresources Utilization of the State Ethnic Affairs Commission-Ministry of Education,School of Life Science,Dalian Nationalities University,Liaoning Dalian 116600,China) |
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| Abstract: | The formation of Schiff base between the modified glycine and pyridoxal-5-phosphate(PLP) on liposome surface was investigated.In HEPES buffer(pH=7.00),the negative charged PLP was attracted by the positive charged head group of peptide lipid and anchored on liposome membrane surface,and then reacted with the amino group of the modified glycine on liposome surface.The UV absorption peak of PLP shifted from 387 nm to 395 nm,when PLP was attached to liposome surface.The absorption peak was changed to 336 nm(Sc... |
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| Keywords: | Schiff base liposome glycine pyridoxal-5-phosphate Cu2 |
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