| 中药活性成分山萘酚与牛血清白蛋白相互作用的研究 |
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| 引用本文： | 欧阳宇,胡艳军,饶小兰.中药活性成分山萘酚与牛血清白蛋白相互作用的研究[J].化学世界,2011,52(8):470-474. |
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| 作者姓名： | 欧阳宇 胡艳军 饶小兰 |
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| 作者单位： | 湖北师范学院化学与环境工程学院,污染物分析与资源化技术湖北省重点实验室,湖北黄石,435002 |
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| 基金项目： | 国家自然科学基金(20803019); 湖北省教育厅项目(Q20082205) |
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| 摘 要： | 应用光谱法研究了中药活性成分山萘酚(Kaempferol)与牛血清白蛋白(BSA)的相互作用,探讨了山萘酚对BSA的荧光猝灭过程的机理,测定了不同温度下该结合反应的结合模式、结合常数、结合位点数、结合热力学参数等；并利用经典位点探针试剂华法林(Warfarin)和布洛芬(Ibuprofen)研究了山萘酚与BSA作用的具...
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| 关 键 词： | 山萘酚 牛血清白蛋白 结合参数 位点探针 光谱法 |
Studies on the Interaction between Kaempferol and Bovine Serum Albumin |
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| OUYANG Yu,HU Yan-jun,RAO Xiao-lan.Studies on the Interaction between Kaempferol and Bovine Serum Albumin[J].Chemical World,2011,52(8):470-474. |
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| Authors: | OUYANG Yu HU Yan-jun RAO Xiao-lan |
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| Affiliation: | OUYANG Yu,HU Yan-jun,RAO Xiao-lan(Hubei Key Laboratory of Pollutant Analysis & Reuse Technology,College of Chemistry and EnvironmentalEngineering,Hubei Normal University,Hubei Huangshi 435002,China) |
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| Abstract: | In this study,the binding of Kaempferol to bovine serum albumin(BSA) was studied at physiological conditions(pH=7.40) by fluorescence and UV-Vis spectroscopies.Kaempferol strongly quenches the intrinsic fluorescence of BSA,and a decrease in the fluorescence quenching constant is observed together with an increase in temperature,which indicates that the fluorescence quenching of BSA by Kaempferol is a result of the formation of Kaempferol-BSA complex.The binding parameters,binding mode,and binding site were ... |
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| Keywords: | Kaempferol bovine serum albumin binding parameters site marker probe spectroscopic methods |
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