Development of a Simple Method for Detecting Presumptive Escherichia coli on Fresh Retail Beef |
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| Authors: | BW Bolling DJ Schmidt SC Ingham |
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| Affiliation: | The authors are with the Dept. of Food Science, Univ. of Wisconsin-Madison, 1605 Linden Dr., Madison, WI53706-1565. Direct inquiries to author Ingham (E-mail: ). |
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| Abstract: | ABSTRACT: A simple method of detecting Escherichia coli on retail beef was developed. Diluent used in sponge-sampling of meat was added to a tube containing double-strength lauryl sulfate tryptone broth + 5-bromo-4-chloro-3-indolyl β-D-glucuronide (X-GlcA) and a Durham tube. Tubes with blue color and gas after 24 h incubation at 35 °C were presumptive-positive. Chi-squared analysis showed a significant ( P < 0.05) relationship between the method and the Petrifilm™ E. coli /Coliform Count Plate method for E. coli in analysis of 66 retail beef samples and the 2 methods had a similar proportion of confirmed isolates (61 and 63%). The method developed is qualitatively comparable to the Petrifilm™ method and provides small-scale beef processors a simple way to monitor sanitary conditions in fabrication and grinding operations. |
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| Keywords: | E coli detection retail beef |
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