Recombinase activating gene expression in thymic subpopulations. A transitional cell type has lost RAG-2 but not RAG-1 |
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Authors: | JJ Campbell Y Hashimoto |
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Affiliation: | University of Pennsylvania, School of Medicine, Department of Pathology, Philadelphia 19104. |
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Abstract: | We have used a highly sensitive Northern blot probing technique to examine RAG-1 and RAG-2 mRNA expression in unmanipulated murine thymocytes of various developmental phenotypes sorted by flow cytometry. We have found that neither RAG-1 nor RAG-2 are down-regulated until thymocytes reach the TCRhi stage. TCRhi, CD8+CD4+ cells have lost RAG-2 mRNA but continue to express significant levels of RAG-1 mRNA, providing molecular evidence that this cell type is transitional between the TCRlo, CD4+CD8+ and the TCRhi, CD4+CD8-, or CD4-CD8+ phenotypes. |
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