外源激素对紫苏愈伤组织诱导及迷迭香酸积累的影响

以紫苏叶片为外植体,通过向B5培养基中添加不同浓度的激素组合,进行正交实验,研究其对紫苏叶片愈伤组织诱导率的影响。结果表明,在B5培养基中,添加2,4-D1.0 mg/L+KT0.4 mg/L+6-BA1.0 mg/L+NAA1.5mg/L时,愈伤组织诱导率最高。通过高效液相色谱法测定,紫苏叶片中迷迭香酸含量为0.25%,而在最佳培养基中培养40 d的愈伤组织中的含量为3.85%,有了显著的提高。研究为紫苏迷迭香酸高产细胞系的建立提供了理论依据。

Effects of External Hormone on Inducing Perilla Frutescens Callus and Accumulation of Rosmarinic Acid

In this study,with the leaves of Perilla frutescens were used as explant,effect of different concentrations of the hormone combination on inducing callus in B5 medium were studied by orthogonal test.It was found that the optimum medium for callus induction is B5 + 2,4- D1.0 mg/L+KT0.4 mg/L+6-BA1.0 mg/L+NAA1.5 mg/L,under the optimum conditions, the highest induction rate was achieved.Furthermore,the content of rosmarinic acid in calli after 40 days culture was achieved at 3.85%,however,the value in the leaves of Perilla frutescens was only 0.25%.The results here provided a theoretical basis for the establishment of high-yielding cell lines.