Comparison of HPLC and Mouse Bioassay Methods for Determining PSP Toxins in Shellfish |
| |
| Authors: | JOHN J SULLIVAN MARTHA G SIMON WAYNE T IWAOKA |
| |
| Affiliation: | Authors Sullivan and Iwaoka are affiliated with the Institute for Food Science &Technology, College of Ocean &Fishery Sciences, Univ. of Washington, Seattle, WA 98195. Author Simon is affiliated with the Dept. of Social and Health Services, State of Washington, Seattle, WA 98104. |
| |
| Abstract: | Paralytic shellfish poisoning (PSP) toxins in shellfish were analyzed and quantified using both a high pressure liquid chromatographic (HPLC) technique and the standard AOAC mouse bioassay. Good correlation between the two assays was obtained at or below the limit of 80 μg toxin/100g, while at higher levels of toxicity, the HPLC technique tended to underestimate total toxicity slightly. Advantages and disadvantages of both techniques are discussed. |
| |
| Keywords: | |
|
|
